![Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-278202415242658.jpg "Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | JE62-71 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human RCAN1 aa 50-150. (Uniprot: P53805) |
| Localization | Cytoplasm, nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RCAN1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for RCAN1 Antibody (NBP3-32100)Find related products by research area.
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| Gene Symbol | RCAN1 |
![Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202410234244.jpg "Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202416372121.jpg "Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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