RB1 Antibody (SPM353) [DyLight 488]

Recombinant human RB1 protein fragment of 283 amino acid residues. (Uniprot: P06400)

aa 703-772

Nuclear

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

IgG1 Kappa

Monoclonal

Mouse

RB1

Protein A or G purified

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Optimal dilution of this antibody should be experimentally determined.