![Western Blot: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Azide-Free-Western-Blot-NBP2-33240-img0003.jpg "Western Blot: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, KD, KO |
| Clone | 172C1094 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen. |
| Specificity | The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for cleaved Retinoblastoma 1 Antibody (NBP2-33240)Find related products by research area.
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| Gene Symbol | RB1 |
![Immunohistochemistry: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, The University of Chicago.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Azide-Free-Immunohistochemistry-NBP2-33240-img0001.jpg "Immunohistochemistry: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, The University of Chicago.")
![Western Blot: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Azide-Free-Western-Blot-NBP2-33240-img0002.jpg "Western Blot: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - Analysis in HL60 cells were treated with camptothecin for 24 hours.")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")