![Western Blot: RAR alpha/NR1B1 Antibody [NBP3-11869] - Human embryonic kidney cells (293T) were transfected with a RAR alpha construct (lane 2) or with a negative control construct (lane 1) and analysed by Western blot using NBP3-11869, diluted 1:750 in BSA/PBS- Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/RAR-alpha-NR1B1-Antibody-Western-Blot-NBP3-11869-img0003.jpg "Western Blot: RAR alpha/NR1B1 Antibody [NBP3-11869] - Human embryonic kidney cells (293T) were transfected with a RAR alpha construct (lane 2) or with a negative control construct (lane 1) and analysed by Western blot using NBP3-11869, diluted 1:750 in BSA/PBS- Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against human RAR alpha/NR1B1 using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein (Uniprot#: P10276). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RARA |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for RAR alpha/NR1B1 Antibody (NBP3-11869)Find related products by research area.
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| Gene Symbol | RARA |
![Chromatin Immunoprecipitation: RAR alpha/NR1B1 Antibody [NBP3-11869] - ChIP assays were performed using NB4 cells, NBP3-11869 and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. This image shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.](http://images.novusbio.com/fullsize/RAR-alpha-NR1B1-Antibody-Chromatin-Immunoprecipitation-NBP3-11869-img0001.jpg "Chromatin Immunoprecipitation: RAR alpha/NR1B1 Antibody [NBP3-11869] - ChIP assays were performed using NB4 cells, NBP3-11869 and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. This image shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.")
![ELISA: RAR alpha/NR1B1 Antibody [NBP3-11869] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of NBP3-11869. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,400.](http://images.novusbio.com/fullsize/RAR-alpha-NR1B1-Antibody-ELISA-NBP3-11869-img0002.jpg "ELISA: RAR alpha/NR1B1 Antibody [NBP3-11869] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of NBP3-11869. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,400.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
