If Ran is not in its GTP form, how to get Ran-GTP? (buffer, reaction time and temperature, GTP kind, etc)

Name: Recombinant Human Ran His Protein
Brand: Novus Biologicals
SKU: NBP1-45255
Price: 409 USD

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We do not anticipate that this protein is active, and it is not recommend for functional assay. NBP1-45255 has been validated in ELISA (for standard curve) and PAGE (protein control) only. If wanting to perform functional assay, the mammalian cell expressed Ran protein will be more suitable. Unfortunately I do not have such information, but can only provide you the general references for you to check: Woo IS., et al. (2008) Apoptosis. 13(10):1223-31 and Ren M., et al. (1994) Mol Cell biol. 14(6):4216-24.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	PAGE
Concentration	1 mg/ml

Description	A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-216 of Human Ran Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL
Source	E. coli
Protein/Peptide Type	Recombinant Protein
Gene	RAN
Purity	>90%, by SDS-PAGE

Dilutions	SDS-Page
Theoretical MW	26.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Purity	>90%, by SDS-PAGE

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