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![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0009.jpg "Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0005.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0007.jpg)
![Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Flow-Cytometry-NBP2-61832-img0002.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0008.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), Hela (6), COS7 (7), and Jurkat (8) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0004.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded cervical cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0006.jpg)
![ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-ELISA-NBP2-61832-img0001.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - RanGTPase detected with Mouse Monoclonal Ran Antibody (8D1A6) in HEK293T and LNCaP C42. Lanes are labeled as follows: 1. Recombinant protein 2. HEK239T 3. HEK293T constitutively expressing GFP-tagged construct 4. LNCaP C42 5. LNCaP C42. Recombinant RAN protein is loaded with HEK293T and LNCaP C42 prostate cell line. Cell lysates run on a 4-15% mini gel. The ladder is Precision Plus Protein Standards Kaleidoscope. Proteins were detected with a secondary anti-mouse conjugated with Alexa Fluor 680 and detected with infrared scanner. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-61832_mouse-monoclonal-ran-antibody-8d1a6-western-blot-162023155342.jpg)
![Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-31776-img0004.jpg "Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-31776] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31776-img0002.jpg)
![Western Blot: Ran Antibody [NBP1-31776] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-31776-img0003.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0006.jpg "Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded rectum cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Immunohistochemistry-NBP2-61840-img0004.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0005.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mouse mAb against A431 (1), C6 (2), Jurkat (3), Hela (4), COS7 (5), and Jurkat (6) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0007.jpg)
![Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded esophageal cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Immunohistochemistry-NBP2-61840-img0003.jpg)
![ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-ELISA-NBP2-61840-img0001.jpg)
![ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-ELISA-NBP2-61840-img0002.jpg)
![Western Blot: Ran Antibody [NB100-1442] - Staining of A431 with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NB100-1442-img0001.jpg "Western Blot: Ran Antibody [NB100-1442] - Staining of A431 with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Ran Antibody [NB100-1442] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-Paraffin-NB100-1442-img0003.jpg)
![Immunohistochemistry-Paraffin: Ran Antibody [NB100-1442] - Staining of paraffin embedded Human Tonsil with antibody at 3 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-Paraffin-NB100-1442-img0002.jpg)
![Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-68870-img0003.jpg "Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-68870] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-68870-img0005.jpg)
![Western Blot: Ran Antibody [NBP1-68870] - Staining of Human Testis lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-68870-img0002.jpg)
![Western Blot: Ran Antibody [NBP2-32280] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32280 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP2-32280-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ran Antibody [NBP2-32280] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32280 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Ran Antibody [NBP2-32280] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32280-img0004.jpg)
![Immunohistochemistry: Ran Antibody [NBP2-32280] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32280-img0003.jpg)
![Immunoprecipitation: Ran Antibody [NBP2-32280] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32280 used for IP at 6 ug per reaction. For blotting immunoprecipitated RAN, NBP2-32280 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunoprecipitation-NBP2-32280-img0002.jpg)
![Western Blot: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP2-32281-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Ran Antibody [NBP2-32281] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32281-img0004.jpg)
![Immunohistochemistry: Ran Antibody [NBP2-32281] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32281-img0003.jpg)
![Immunoprecipitation: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for IP at 6 ug per reaction. For blotting immunoprecipitated RAN, NBP2-32281 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunoprecipitation-NBP2-32281-img0002.jpg)
![Western Blot: Ran Antibody (ARAN1) [NB600-1082] - Whole extract of Jurkat cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Ran Clone: ARAN1 and developed using Goat Anti-Mouse IgG-ALP and a NBT/BCIP substrate.](http://images.novusbio.com/fullsize/Ran-Antibody-ARAN1-Western-Blot-NB600-1082-img0001.jpg "Western Blot: Ran Antibody (ARAN1) [NB600-1082] - Whole extract of Jurkat cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Ran Clone: ARAN1 and developed using Goat Anti-Mouse IgG-ALP and a NBT/BCIP substrate.")
![Immunohistochemistry: Ran Antibody (15A8) [NBP3-26352] - Image of Ran Antibody (15A8) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB.](http://images.novusbio.com/fullsize/nbp3-26352_rabbit-ran-mab-15a8-262202414471545.jpg "Immunohistochemistry: Ran Antibody (15A8) [NBP3-26352] - Image of Ran Antibody (15A8) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody (15A8) [NBP3-26352] - Staining of Hela with Ran Antibody (15A8) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 527-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26352_rabbit-ran-mab-15a8-28220241312242.jpg)
![Flow Cytometry: Ran Antibody (15A8) [NBP3-26352] - Overlay Peak curve showing Hela cells stained with Ran Antibody (15A8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26352_rabbit-ran-mab-15a8-282202414312723.jpg)
![Western Blot: Ran Antibody (SR1381) [NBP3-21795] - Western blot analysis using NBP3-21795 in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21795_rabbit-ran-mab-sr1381-5720231611215.jpg "Western Blot: Ran Antibody (SR1381) [NBP3-21795] - Western blot analysis using NBP3-21795 in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.")
![Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: LoVo cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: MDA-MB-231 cell lysate (15 ug/Lane) Lane 7: MCF7 cell lysate (15 ug/Lane) Lane 8: RAW264.7 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: Rat thymus tissue lysate (30 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32892_rabbit-ran-mab-je33-28-288202412442969.jpg "Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: LoVo cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: MDA-MB-231 cell lysate (15 ug/Lane) Lane 7: MCF7 cell lysate (15 ug/Lane) Lane 8: RAW264.7 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: Rat thymus tissue lysate (30 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Ran Antibody (JE33-28) [NBP3-32892] - Flow cytometric analysis of HeLa cells labeling Ran. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32892, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32892_rabbit-ran-mab-je33-28-392024884630.jpg)
![Western Blot: Ran Antibody (6S5X5) [NBP3-16393] - Western blot analysis of extracts of various cell lines, using Ran Rabbit mAb (NBP3-16393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Ran-Antibody-6S5X5-Western-Blot-NBP3-16393-img0001.jpg "Western Blot: Ran Antibody (6S5X5) [NBP3-16393] - Western blot analysis of extracts of various cell lines, using Ran Rabbit mAb (NBP3-16393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")