![Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-68870-img0003.jpg "Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-YEHDLEVAQTTALP corresponding to C-Terminus according to NP_006316.1. |
| Epitope | C-YEHDLEVAQTTALP |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RAN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 25 kDa band observed in human, mouse and rat testis lysates (calculated MW of 24.4 kDa band according to NP_006316.1). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-68870 | Applications | Species |
|---|---|---|
| Ly TK, Wang J, Pereira R et al. Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation. J Biol Chem 2010-02-01 [PMID: 20028979] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Ran Antibody (NBP1-68870)Find related products by research area.
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Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD) Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm. Transportin 1 is co... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RAN |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-68870] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-68870-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-68870] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: Ran Antibody [NBP1-68870] - Staining of Human Testis lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-68870-img0002.jpg "Western Blot: Ran Antibody [NBP1-68870] - Staining of Human Testis lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
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