![Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Western-Blot-NBP2-15085-img0015.jpg "Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RAB11B. The exact sequence is proprietary. |
| Predicted Species | Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAB11B |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 24 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-15085 | Applications | Species |
|---|---|---|
| Kallergi E, Daskalaki AD, Kolaxi A et al. Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice Nature communications 2022-02-03 [PMID: 35115539] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for RAB11B Antibody (NBP2-15085)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: RAB11B Antibody [NBP2-15085] - DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB11B stained by RAB11B antibody [N1C3] diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15085-img0017.jpg "Immunocytochemistry/Immunofluorescence: RAB11B Antibody [NBP2-15085] - DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB11B stained by RAB11B antibody [N1C3] diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0018.jpg "Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: RAB11B Antibody [NBP2-15085] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Western-Blot-NBP2-15085-img0013.jpg "Western Blot: RAB11B Antibody [NBP2-15085] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RAB11B Antibody [NBP2-15085] - HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min. Green.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15085-img0006.jpg "Immunocytochemistry/Immunofluorescence: RAB11B Antibody [NBP2-15085] - HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min. Green.")
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Human colon carcinoma, using RAB11B antibody antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0002.jpg "Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Human colon carcinoma, using RAB11B antibody antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0008.jpg "Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Frozen: RAB11B Antibody [NBP2-15085] - Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Frozen-NBP2-15085-img0010.jpg "Immunohistochemistry-Frozen: RAB11B Antibody [NBP2-15085] - Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0011.jpg "Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0016.jpg "Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: RAB11B Antibody [NBP2-15085] - Proteomic profiling of autophagic cargo during NMDAR-LTD.a–d WB analyses of different fractions along the autophagic vesicle purification procedure, using antibodies against a autophagosomal markers (LC3B, p62, Atg16L1, & Atg9A), b ER-Golgi markers (TGN, LMAN1, SAR1a), c endosomal markers (Rab11b, EEA1), & d markers of plasma-membrane (Stx4), extracellular vesicles (Alix) & nuclear extracts (TBP). N = 3 independent experiments. e Graph showing the cell component analysis, as false discovery rate (FDR)-corrected p-values, of dynamic cargo (total of 393 proteins) that is enriched (up) or less abundant (down) in AVs after LTD, compared to control. f Graphical representation of proteins enriched in AVs upon LTD, w/ relation to synapse. g WB analysis of PK-treated control & LTD-AVs, validating the presence of proteins identified by the proteomic analyses in autophagic vesicles. Postsynaptic density (PSD) fraction used as reference. Graph showing the fold change of normalized levels of proteins validated by WB, as a ratio of LTD to control. Cargo proteins normalized to levels of p62, which remains unaffected at the early phase of LTD. N = 3 independent AV preparations. Bars represent mean values ± SEM. Statistical analysis performed using paired, two-tailed Student’s t-test (GluA1,N = 6, P = 0.0002; GluA2, N = 6, P = 0.0039; Pick1, N = 5, P = 0.011; SAP97, N = 5, P = 0.0179; FYN, N = 8, P Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-91020242014691.jpg "Western Blot: RAB11B Antibody [NBP2-15085] - Proteomic profiling of autophagic cargo during NMDAR-LTD.a–d WB analyses of different fractions along the autophagic vesicle purification procedure, using antibodies against a autophagosomal markers (LC3B, p62, Atg16L1, & Atg9A), b ER-Golgi markers (TGN, LMAN1, SAR1a), c endosomal markers (Rab11b, EEA1), & d markers of plasma-membrane (Stx4), extracellular vesicles (Alix) & nuclear extracts (TBP). N = 3 independent experiments. e Graph showing the cell component analysis, as false discovery rate (FDR)-corrected p-values, of dynamic cargo (total of 393 proteins) that is enriched (up) or less abundant (down) in AVs after LTD, compared to control. f Graphical representation of proteins enriched in AVs upon LTD, w/ relation to synapse. g WB analysis of PK-treated control & LTD-AVs, validating the presence of proteins identified by the proteomic analyses in autophagic vesicles. Postsynaptic density (PSD) fraction used as reference. Graph showing the fold change of normalized levels of proteins validated by WB, as a ratio of LTD to control. Cargo proteins normalized to levels of p62, which remains unaffected at the early phase of LTD. N = 3 independent AV preparations. Bars represent mean values ± SEM. Statistical analysis performed using paired, two-tailed Student’s t-test (GluA1,N = 6, P = 0.0002; GluA2, N = 6, P = 0.0039; Pick1, N = 5, P = 0.011; SAP97, N = 5, P = 0.0179; FYN, N = 8, P Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-91020242014663.jpg "Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
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![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")