![Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining in human testis and endometrium tissues using anti-RAB11FIP5 antibody. Corresponding RAB11FIP5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RAB11FIP5-Antibody-Immunohistochemistry-Paraffin-NBP1-81855-img0011.jpg "Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining in human testis and endometrium tissues using anti-RAB11FIP5 antibody. Corresponding RAB11FIP5 RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SPHPVKPLSAAPVEGSPDRKQSRSSLSIALSSGLEKLKTVTSGSIQPVTQAPQAGQMVDTKRLKDSAVLDQSAKYYHLTHDELISLLLQRERELSQRDEHVQEL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAB11FIP5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-81855 | Applications | Species |
|---|---|---|
| Solinger JA, Rashid HO, Prescianotto-Baschong C, Spang A FERARI is required for Rab11-dependent endocytic recycling Nat. Cell Biol. 2020-01-27 [PMID: 31988382] (WB, KO, Human) | WB, KO | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: RAB11FIP5 Antibody [NBP1-81855] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/RAB11FIP5-Antibody-Western-Blot-NBP1-81855-img0006.jpg "Western Blot: RAB11FIP5 Antibody [NBP1-81855] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: RAB11FIP5 Antibody [NBP1-81855] - Staining of human cell line A-431 shows localization to microtubule organizing center & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RAB11FIP5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81855-img0008.jpg "Immunocytochemistry/Immunofluorescence: RAB11FIP5 Antibody [NBP1-81855] - Staining of human cell line A-431 shows localization to microtubule organizing center & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/RAB11FIP5-Antibody-Immunohistochemistry-Paraffin-NBP1-81855-img0009.jpg "Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining of human endometrium shows low expression as expected.")
![Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RAB11FIP5-Antibody-Immunohistochemistry-Paraffin-NBP1-81855-img0010.jpg "Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining of human testis shows high expression.")
![Holo-Transferrin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Holo-Transferrin [Unconjugated]")
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")