Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [DyLight 405]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Pyruvate dehydrogenase (PDH) complex is localized to mitochondrial matrix, wherein it catalyzes the irreversible oxidative decarboxylation of pyruvate to generate acetyl-CoA, NADH, and CO2. The PDH complex contains three primary enzyme components: pyruvate dehydrogenase (E1 or PDHA- heterotetramer of two alpha- and two beta-subunits), dihydrolipoamide transacetylase (E2 or DLAT), and dihydrolipoamide dehydrogenase (E3 or DLD). The activity of PDH is controlled by specific E1 kinase and phospho-E1-phosphatase enzymes, which respectively inactivate and activate PDH complex by phosphorylation and dephosphorylation of E1alpha-subunit's serine residues. PDK family kinases mediated phosphorylation at Ser-293 blocks the access to active site leading to inactivation of the enzyme and vice versa. E3-binding protein (E3BP) is another component which is required for proper interaction of E2 and E3 components, and the overall complex contains 12 copies of E3, 30 copies of E1, 60 copies of E2, and 12 copies of E3BP. Acetyl-CoA generated gets utilized in TCA (citric acid/Krebs cycle) where it reacts with oxaloacetate to form citrate or it may also be used for biosynthesis of fatty acid or cholesterol. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD), X-linked Leigh syndrome (X-LS) and it has also been implicated in ageing, glucose intolerance, cancer as well as Alzheimer's disease.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Array NB110-93479V

• Pyruvate Dehydrogenase E1-alpha subunit Antibodies
• Pyruvate Dehydrogenase E1-alpha subunit ELISA Kits
• Pyruvate Dehydrogenase E1-alpha subunit Lysate
• Pyruvate Dehydrogenase E1-alpha subunit Proteins