Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free

Pyruvate dehydrogenase (PDH) complex is localized to mitochondrial matrix, wherein it catalyzes the irreversible oxidative decarboxylation of pyruvate to generate acetyl-CoA, NADH, and CO2. The PDH complex contains three primary enzyme components: pyruvate dehydrogenase (E1 or PDHA- heterotetramer of two alpha- and two beta-subunits), dihydrolipoamide transacetylase (E2 or DLAT), and dihydrolipoamide dehydrogenase (E3 or DLD). The activity of PDH is controlled by specific E1 kinase and phospho-E1-phosphatase enzymes, which respectively inactivate and activate PDH complex by phosphorylation and dephosphorylation of E1alpha-subunit's serine residues. PDK family kinases mediated phosphorylation at Ser-293 blocks the access to active site leading to inactivation of the enzyme and vice versa. E3-binding protein (E3BP) is another component which is required for proper interaction of E2 and E3 components, and the overall complex contains 12 copies of E3, 30 copies of E1, 60 copies of E2, and 12 copies of E3BP. Acetyl-CoA generated gets utilized in TCA (citric acid/Krebs cycle) where it reacts with oxaloacetate to form citrate or it may also be used for biosynthesis of fatty acid or cholesterol. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD), X-linked Leigh syndrome (X-LS) and it has also been implicated in ageing, glucose intolerance, cancer as well as Alzheimer's disease.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: FLOW, IB, IF/IHC, IHC-Fr, IHC-FrFl, IP, KO, WB.

Showing Publications 1 - 10 of 57. Show All 57 Publications. Collapse Publications.

Publications using NB110-93479	Applications	Species
Li Y, Cai J, Liu Y et al. CcpA-Knockout Staphylococcus aureus Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice Pathogens 2023-10-30 [PMID: 38003764] (WB, Mouse)	WB	Mouse
Huo J, Prasad V, Grimes KM et al. MCUb is an inducible regulator of calcium-dependent mitochondrial metabolism and substrate utilization in muscle Cell reports 2023-11-28 [PMID: 37976157]
Rossi SP, Matzkin ME, Riviere E et al. Melatonin improves oxidative state and lactate metabolism in rodent Sertoli cells Molecular and cellular endocrinology 2023-07-27 [PMID: 37516434] (WB, Human) Details: 1:1000 WB dilution	WB	Human
Bekeova C, Han JI, Xu H et al. Acyl-CoA thioesterase-2 facilitates ?-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner bioRxiv : the preprint server for biology 2023-06-27 [PMID: 37425757] (WB, Mouse)	WB	Mouse
Li J, Zhu X, Oberdier MT et al. A cell-penetrating PHLPP peptide improves cardiac arrest survival in murine and swine models The Journal of clinical investigation 2023-05-01 [PMID: 37115695] (WB, Mouse)	WB	Mouse
Holloway C, Zhong G, Kim YK et al. Retinoic acid regulates pyruvate dehydrogenase kinase 4 (Pdk4) to modulate fuel utilization in the adult heart: Insights from wild-type and beta-carotene 9',10' oxygenase knockout mice FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-09-01 [PMID: 36004605] (WB, Mouse)	WB	Mouse
Ito N, Takatsu A, Ito H et al. Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging Cell reports 2022-07-26 [PMID: 35905718] (WB, Mouse) Details: 1:4000 dilution	WB	Mouse
Li J, Chang WT, Qin G et al. Baicalein Preconditioning Cardioprotection Involves Pro-Oxidant Signaling and Activation of Pyruvate Dehydrogenase The American journal of Chinese medicine 2022-06-23 [PMID: 35748215]
Martin A, Fernandez M, Cattaneo E et al. Type 1 Insulin-Like Growth Factor Receptor Nuclear Localization in High-Grade Glioma Cells Enhances Motility, Metabolism, and In Vivo Tumorigenesis Frontiers in Endocrinology [PMID: 35574033] (WB, Human)	WB	Human
Gatie M, Cooper T, Khazaee R et al. Lactate enhances mouse ES cell differentiation towards XEN cells in vitro Stem Cells 2022-03-24 [PMID: 35323987]
Damerau A, Kirchner M, Pfeiffenberger M et al. Metabolic reprogramming of synovial fibroblasts in osteoarthritis by inhibition of pathologically overexpressed pyruvate dehydrogenase kinases Metabolic engineering 2022-03-13 [PMID: 35296430] (FLOW, Human)	FLOW	Human
Kanan Y, Hackett SF, Taneja K et al. Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa Free radical biology & medicine 2022-03-01 [PMID: 35134532] (WB, Mouse)	WB	Mouse
Burns Je, Hurst Cd, Knowles Ma Et Al. The Warburg effect as a therapeutic target for bladder cancers and intratumoral heterogeneity in associated molecular targets Cancer science 2021-06-28 [PMID: 34181805] (WB)	WB
Vitvitsky V, Kumar R, Libiad M et al. The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis The Journal of biological chemistry 2021-04-29 [PMID: 33933447] (WB)	WB
Santiappillai NT, Abuhammad S, Slater A et al. CDK4/6 Inhibition Reprograms Mitochondrial Metabolism in BRAFV600 Melanoma via a p53 Dependent Pathway Cancers 2021-01-29 [PMID: 33572972] (WB, Human)	WB	Human
Hjelholt, A J, Charidemou, E Et al. Insulin resistance induced by growth hormone is linked to lipolysis and associated with suppressed pyruvate dehydrogenase activity in skeletal muscle: a 2 x 2 factorial, randomised, crossover study in human individuals. Diabetologia 2020-12-01 [PMID: 32945898] (IF/IHC, Human)	IF/IHC	Human
Na YR, Jung D, Song J et al. Pyruvate dehydrogenase kinase is a negative regulator of interleukin-10 production in macrophages J Mol Cell Biol 2020-01-03 [PMID: 31900478] (FLOW, Mouse)	FLOW	Mouse
Jebessa ZH, Shanmukha Kumar D, Dewenter M et al. The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4 Nat Metab 2019-11-01 [PMID: 31742248] (IB, KO, Human, Mouse, Rat)	IB, KO	Human, Mouse, Rat
Lu H, Lu Y, Xie Y, et al. Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma. JCI Insight. 2019-10-03 [PMID: 31578313] (WB, Human)	WB	Human
Jiang H, Jin J, Duan Y et al. Mitochondrial Uncoupling Coordinated With PDH Activation Safely Ameliorates Hyperglycemia Via Promoting Glucose Oxidation Diabetes 2019-08-30 [PMID: 31471292]
Li J, Zhu X, Wang H et al. Akt1-mediated CPR cooling protection targets regulators of metabolism, inflammation and contractile function in mouse cardiac arrest PLoS ONE 2019-08-09 [PMID: 31398213] (WB, Mouse)	WB	Mouse
Thapa D, Zhang M, Manning JR Loss of GCN5L1 in cardiac cells limits mitochondrial respiratory capacity under hyperglycemic conditions Physiol Rep 2019-04-01 [PMID: 31033247] (WB, Rat)	WB	Rat
Zhong W, Xu M, Li C et al. ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCb3 Catalysis: Targeting It Eradicates Leukemia Stem Cells Cell Rep 2019-02-19 [PMID: 30784597] (WB, Human)	WB	Human
Zhuang Y, Ly RC, Frazier CV et al. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer. Cancer Metab 2019-01-24 [PMID: 30697423] (WB, Human)	WB	Human
Kwong JQ, Huo J, Bround MJ et al. The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle JCI Insight 2018-11-15 [PMID: 30429366] (WB, Mouse)	WB	Mouse
Deng YH, Deng ZH, Hao H et al. MicroRNA-23a promotes colorectal cancer cell survival by targeting PDk4. Exp. Cell Res. 2018-10-18 [PMID: 30342991] (WB, Human)	WB	Human
Thapa D, Wu k, Stoner MW et al. The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial B-oxidation enzyme HADHA. J. Biol. Chem. 2018-10-15 [PMID: 30323061] (WB, Mouse)	WB	Mouse
Lemecha M, Morino K, Imamura T et al. MiR-494-3p regulates mitochondrial biogenesis and thermogenesis through PGC1-a signalling in beige adipocytes Acta Biomater 2018-10-11 [PMID: 30305668] (WB, Mouse)	WB	Mouse
Gao Y, Zhou Z, Ren T et al. Alcohol inhibits T-cell glucose metabolism and hepatitis in ALDH2-deficient mice and humans: roles of acetaldehyde and glucocorticoids. Gut 2018-08-18 [PMID: 30121625] (WB, Human)	WB	Human
Menk AV, Scharping NE, Moreci RS et al. Early TCR Signaling Induces Rapid Aerobic Glycolysis Enabling Distinct Acute T Cell Effector Functions Cell Rep 2018-02-06 [PMID: 29425506] (WB, IP, Mouse)	WB, IP	Mouse
Butyrate induces apoptosis by activating PDC and inhibiting complex I through SIRT3 inactivation. Xu S, Liu C, Xu W et al. Signal Transduct Target Ther [PMID: 29263907] (WB, Human)	WB	Human
Liu X, Zuo R, Bao Y et al. Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts Sci Rep 2017-08-16 [PMID: 28814762] (WB, Human)	WB	Human
Gorga A, Rindone GM, Regueira M et al. PPARgamma activation regulates lipid droplet formation and lactate production in rat Sertoli cells. Cell Tissue Res. 2017-04-21 [PMID: 28432465]
Bhosale G, Sharpe J, Koh A et al. Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics Biochim. Biophys. Acta 2017-01-26 [PMID: 28132899] (WB, Human)	WB	Human
Jun JC, Devera R, Unnikrishnan D et al. Adipose HIF-1alpha causes obesity by suppressing brown adipose tissue thermogenesis. J Mol Med (Berl) 2017-03-01 [PMID: 27738746]
Zhong W, Yi Q, Xu B et al. ORP4L is essential for T-cell acute lymphoblastic leukemia cell survival. Nat Commun 2016-09-01 [PMID: 27581363] (WB)	WB
Lu H, Li X, Lu Y et al. ASCT2 (SLC1A5) is an EGFR-associated protein that can be co-targeted by cetuximab to sensitize cancer cells to ROS-induced apoptosis Cancer Lett. 2016-07-19 [PMID: 27450723] (IP, WB)	IP, WB
Rossi SP, Windschuettl S, Matzkin ME et al. Reactive oxygen species (ROS) production triggered by prostaglandin D2 (PGD2) regulates lactate dehydrogenase (LDH) expression/activity in TM4 Sertoli cells. Mol. Cell. Endocrinol. 2016-06-18 [PMID: 27329155] (WB, Mouse)	WB	Mouse
Regueira M, Artagaveytia SL, Galardo MN et al. Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells. Reproduction. 2015-07-29 [PMID: 26224098] (WB, Rat) Details: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody recognizing the phosphorylated form of PDC (P-PDC) was used for WB analysis of lysates from Sprague–Dawley rats' Sertoli cells that were stimulated in vitro with follicle-stimulating hormone or basic fibroblast growth factor (Figure 6).	WB	Rat
Zuo RJ, Gu XW, Qi QR et al. Warburg-like glycolysis and lactate shuttle in female mouse decidua during early pregnancy J. Biol. Chem. 2015-07-15 [PMID: 26178372] (WB, Mouse) Details: p-Pdhe1-alpha [p Ser293] antibody used for Western blot analysis of primary mouse endometrial stromal cells.	WB	Mouse
Hagopian K, Tomilov AA, Kim K et al. Key Glycolytic Enzyme Activities of Skeletal Muscle Are Decreased under Fed and Fasted States in Mice with Knocked Down Levels of Shc Proteins PLoS ONE 2015-04-17 [PMID: 25880638] (Mouse)		Mouse
Llorente-Folch I, Rueda CB, Pardo B et al. The regulation of neuronal mitochondrial metabolism by calcium. J. Physiol. (Lond.). 2015-03-23 [PMID: 25809592] (WB, Mouse)	WB	Mouse
Rellinger EJ, Romain C, Choi S et al. Silencing gastrin-releasing peptide receptor suppresses key regulators of aerobic glycolysis in neuroblastoma cells Pediatr Blood Cancer. 2015-01-28 [PMID: 25630799] (WB, Human)	WB	Human
Ho N, Coomber BL. Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells. Exp Cell Res 2015-02-01 [PMID: 25536473] (Human)		Human
Heiss EH, Kramer MP, Atanasov AG et al. Glycolytic Switch in Response to Betulinic Acid in Non-Cancer Cells PLoS One. 2014-12-21 [PMID: 25531780] (WB, Mouse)	WB	Mouse
Tomilov A, Bettaieb A, Kim K et al. Shc depletion stimulates brown fat activity in vivo and in vitro. Aging Cell. 2014-09-26 [PMID: 25257068]
Regueira M, Riera MF, Galardo MN et al. Activation of PPAR alpha and PPAR beta/delta regulates Sertoli cell metabolism. Mol Cell Endocrinol. 2013-10-12 [PMID: 24128860] (WB, Rat)	WB	Rat
Contractor T, Harris CR. p53 negatively regulates transcription of the pyruvate dehydrogenase kinase Pdk2. Cancer Research. 2011-11-28 [PMID: 22123926]
Grassian AR, Metallo CM, Coloff JL et al. Erk regulation of pyruvate dehydrogenase flux through PDK4 modulates cell proliferation. Genes Dev;25(16):1716-1733. 2011-08-15 [PMID: 21852536]
Puthanveetil P, Wang Y, Wang F et al. The increase in cardiac pyruvate dehydrogenase kinase-4 after short-term dexamethasone is controlled by an Akt-p38-forkhead box other factor-1 signaling axis. Endocrinology;151(5):2306-18. 2010-05-01 [PMID: 20181797] (WB, Rat)	WB	Rat
Acin-Perez R, Hoyos B, Zhao F et al. Control of oxidative phosphorylation by vitamin A illuminates a fundamental role in mitochondrial energy homoeostasis. FASEB J :fj.09-142281. 2009-01-01 [PMID: 19812372]
Abdon B The Role of Endoplasmic Reticulum Associated Degradation in Skeletal Muscle Growth and Metabolism Thesis 2023-01-01 (IHC-FrFl, IHC-Fr, Mouse)	IHC-FrFl, IHC-Fr	Mouse
Delaney Lm, Ho N, Morrison J et al. Dichloroacetate affects proliferation but not survival of human colorectal cancer cells. Apoptosis. 2014-10-26 [PMID: 25344893] (WB, Human)	WB	Human
Wu CA, Chao Y, Shiah SG, Lin WW. Nutrient deprivation induces the Warburg effect through ROS/AMPK-dependent activation of pyruvate dehydrogenase kinase. Biochim Biophys Acta 2013-01-31 [PMID: 23376776] (WB, Human)	WB	Human
Gong J, Hoyos B, Acin-Perez R, Vinogradov V et al. Two protein kinase C isoforms, delta and epsilon, regulate energy homeostasis in mitochondria by transmitting opposing signals to the pyruvate dehydrogenase complex FASEB J 2012-08-01 [PMID: 22573912] (WB, Mouse)	WB	Mouse
Acin-Perez R, Hoyos B, Gong J et al. Regulation of intermediary metabolism by the PKCdelta signalosome in mitochondria FASEB J 2010-12-01 [PMID: 20798245] (WB, Mouse)	WB	Mouse
McFate, T et al. J. Bio. Chem. 283(33):22700-22708. 2008-01-01 [PMID: 18541534]
Show All 57 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	07/10/2018	View Summary Application Western Blot Sample Tested placenta cells Species Human Lot A1
Enlarge	4 reviewed by: Shahreen Amin	WB	Human	06/12/2018	View Summary Application Western Blot Sample Tested Differentiated Neuronal NTERA2 cells,NTera-2 human testicular embryonic carcinoma cell line Species Human Lot p-1 Comments Comments The site was similar in PDHA2 and we IP PDHA2 and its mutants at the site and probed with the antibody
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	05/12/2016	View Summary Application Western Blot Sample Tested primary mouse macrophage lysates Species Mouse
Enlarge	5 reviewed by: Silvina Meroni	WB	Rat	01/14/2014	View Summary Application Western Blot Sample Tested rat Sertoli cells Species Rat Lot K-1
Enlarge	5 reviewed by: Alexandra Grassian	WB	Human	08/12/2010	View Summary Application Western Blot Sample Tested MCF10A whole cell lysate, Sample Amount: 30ug Species Human

Array NB110-93479

• Pyruvate Dehydrogenase E1-alpha subunit Antibodies
• Pyruvate Dehydrogenase E1-alpha subunit ELISA Kits
• Pyruvate Dehydrogenase E1-alpha subunit Lysate
• Pyruvate Dehydrogenase E1-alpha subunit Proteins