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Recombinant Human PTTG1 His Protein

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SDS-Page: Securin Protein [NBP1-30200] - PTTG1, 24.1 kDa (222aa), confirmed by MALDI-TOF with a purity of 80% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

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Recombinant Human PTTG1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-202 of Human Securin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PTTG1
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
24.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 20% glycerol, 1 mM EDTA, 0.1 mM PMSF
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PTTG1 His Protein

  • EAP1MGC126883
  • ESP1-associated protein 1
  • Esp1-associated protein
  • hPTTG
  • pituitary tumor-transforming 1
  • Pituitary tumor-transforming gene 1 protein
  • PTTGMGC138276
  • securin
  • Tumor-transforming protein 1
  • TUTR1HPTTG

Background

PTTG1, also known as Securin, is primarily involved in the regulation of sister chromatid separation during cell division. It has two identified roles; the first one is to help the transport of separase(cysteine protease) to the nucleus and the second role is to inhibit the catalytic activity of separase. It is ubiquitinated by the Anaphase Promoting Complex (APC), and then degraded by the Proteasome, releasing separase. Recombinant PTTG1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Securin Recombinant Protein (NBP1-30200) (0)

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Bioinformatics

Gene Symbol PTTG1