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PTK7/CCK4 Antibody [Unconjugated]

 (9 Publications)
 (1 Review)
 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 171 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4499-SP
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PTK7/CCK4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308
Specificity
Detects human, mouse and rat PTK7/CCK4 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTK7
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
AF4499 in the following applications:
Publications
Read Publications using
AF4499 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTK7/CCK4 Antibody [Unconjugated]

  • CCK4
  • CCK-4
  • CCK4Colon carcinoma kinase 4
  • colon carcinoma kinase-4
  • Protein-tyrosine kinase 7
  • Pseudo tyrosine kinase receptor 7
  • PTK7 protein tyrosine kinase 7
  • PTK7
  • Tyrosine-protein kinase-like 7

Background

CCK4 (colon carcinoma kinase 4, also known as PTK7) is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, CCK4 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTK7/CCK4 Antibody (AF4499)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, Neutralization, Western Blot.


Filter By Application
ICC
(2)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF4499 Applications Species
MT Lam Wong, TL Wong, L Zhou, K Man, J Purcell, TK Lee, JP Yun, S Ma Protein tyrosine kinase 7 (PTK7) promotes metastasis in hepatocellular carcinoma via SOX9 regulation and TGF-beta signaling Cellular and Molecular Gastroenterology and Hepatology, 2022-10-04;0(0):. 2022-10-04 [PMID: 36202326] (Western Blot, Human) Western Blot Human
Anna Paola Carreca, Veronica Maria Pravatà, Danilo D’Apolito, Simone Bonelli, Matteo Calligaris, Elisa Monaca et al. Quantitative Proteomics Reveals Changes Induced by TIMP-3 on Cell Membrane Composition and Novel Metalloprotease Substrates International Journal of Molecular Sciences 2021-02-27 [PMID: 33673623]
T Osthues, B Zimmer, V Rimola, K Klann, K Schilling, P Mathoor, C Angioni, A Weigert, G Geisslinge, C Münch, K Scholich, M Sisignano The Lipid Receptor G2A (GPR132) Mediates Macrophage Migration in Nerve Injury-Induced Neuropathic Pain Cells, 2020-07-21;9(7):. 2020-07-21 [PMID: 32708184] (Neutralization, Mouse) Neutralization Mouse
Fontanals-Cirera B, Hasson D, Vardabasso C et al. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Mol Cell 11/16/2017 [PMID: 29149598]
Golubkov V, Prigozhina N, Zhang Y, Stoletov K, Lewis J, Schwartz P, Hoffman R, Strongin A Protein-tyrosine pseudokinase 7 (PTK7) directs cancer cell motility and metastasis. J Biol Chem, 2014-07-08;289(35):24238-49. 2014-07-08 [PMID: 25006253] (Western Blot, Human) Western Blot Human
Golubkov V, Strongin A Downstream signaling and genome-wide regulatory effects of PTK7 pseudokinase and its proteolytic fragments in cancer cells. Cell Commun Signal, 2014-03-11;12(0):15. 2014-03-11 [PMID: 24618420] (Western Blot, Human) Western Blot Human
Golubkov V, Strongin A Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7). J Biol Chem, 2012-10-24;287(50):42009-18. 2012-10-24 [PMID: 23095747] (ICC, Western Blot, Human) ICC, Western Blot Human
Golubkov VS, Aleshin AE, Strongin AY Potential relation of aberrant proteolysis of human protein tyrosine kinase 7 (PTK7) chuzhoi by membrane type 1 matrix metalloproteinase (MT1-MMP) to congenital defects. J. Biol. Chem., 2011-04-25;286(23):20970-6. 2011-04-25 [PMID: 21518755] (ICC, Human) ICC Human
Lee HK, Chauhan SK, Kay E Flt-1 regulates vascular endothelial cell migration via a protein tyrosine kinase-7-dependent pathway. Blood, 2011-04-01;117(21):5762-71. 2011-04-01 [PMID: 21460247] (Western Blot, Human) Western Blot Human

Review for PTK7/CCK4 Antibody (AF4499) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF4499:
Filter by Applications
WB
(1)
All Applications
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23095747
SpeciesHuman

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Recent Reviews

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 Verified Customer
01/08/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol PTK7
Uniprot