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Prox1 Antibody (5G10) [Alexa Fluor® 647]

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Flow Cytometry: Prox1 Antibody (5G10) [Alexa Fluor® 647] [NBP1-30045AF647] - An intracellular stain was performed on HepG2 cells with Prox1 [5G10] Antibody NBP1-30045AF647 (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, CyTOF-ready, IHC
Clone
5G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

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Prox1 Antibody (5G10) [Alexa Fluor® 647] Summary

Immunogen
The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786]
Epitope
The homeo prospero domain of Prox1
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PROX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

It is expected to work with all vertebrates including mammals, birds and fish. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Prox1 Antibody (5G10) [Alexa Fluor® 647]

  • Homeobox prospero-like protein PROX1
  • prospero homeobox 1
  • prospero homeobox protein 1
  • prospero-related homeobox 1
  • Prox1
  • PROX-1

Background

PROX1 may play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons. PROX1 antibodies are also used as a highly specific marker of lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Prox1 Antibody (NBP1-30045AF647). (Showing 1 - 1 of 1 FAQ).

  1. Which isotype ctrl would you recommend for NBP1-30045AF647?
    • NBP1-30045AF647 is a mouse monoclonal with isotype subclass of IgG1 kappa. This means you will need a mouse IgG1 kappa isotype control that is conjugated to AF647 (for more information on isotype controls and choosing them, please use our excellent resource if you are interested).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PROX1