Home » Protein Kinase A Regulatory Subunit II alpha » Protein Kinase A Regulatory Subunit II alpha Antibodies » Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated]

Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated]

 (1 Publication)
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKA RIIa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more
PKA RIIa was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were ...read more
PKA RII alpha was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
2394D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8000-SP
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Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide containing Human/Mouse PKA RII alpha
Accession # P13861
Specificity
Detects human PKA RII alpha in direct ELISAs. Detects human and mouse PKA RII alpha in Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.3-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using MAB8000.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated]

  • cAMP-dependent protein kinase regulatory subunit RII alpha
  • cAMP-dependent protein kinase type II-alpha regulatory subunit
  • PKA RII alpha
  • PKR2
  • PRKAR2
  • PRKAR2A
  • protein kinase A, RII-alpha subunit
  • protein kinase, cAMP-dependent, regulatory subunit type II alpha

Background

PRKAR2 is a regulatory subunit of the cAMP-dependent protein kinases involved in CAMP signaling in cells.  Four types of regulatory chains are found and their expression varies among tissues.  In some cases expression is constitutive and in other cases it is inducible.  PRKAR2 seems to be involved in both endosome-to-Golgi and Golgi-to-ER transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protein Kinase A
Regulatory Subunit II ...
MAB8000
Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated]
Species: Hu, Mu
Applications: WB, IHC, ICC/IF

Publications for Protein Kinase A Regulatory Subunit II alpha Antibody (MAB8000)(1)

We have publications tested in 1 confirmed species: Human.


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