Home » Protein Disulfide Isomerase/P4HB » Protein Disulfide Isomerase/P4HB ELISA Kits » Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)
Novus Biologicals products are now on bio-techne.com

Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325] - Example standard curve for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325] - Example standard curve for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric). These standard curves are provided for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP
Suitable Sample Type
Lysate
Standard Curve Range
125-8000 pg/ml (example only; lot dependent)
Sensitivity
42.02 pg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) Summary

Specificity
Recognizes both recombinant and natural Human Protein Disulfide Isomerase/P4HB.
Standard Curve Range
125-8000 pg/ml (example only; lot dependent)
Sensitivity
42.02 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 5.3 %
Intra-Assay
CV% < 3.2
Spike Recovery
78-82%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
P4HB

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. 1. 96 well microplate coated with Capture Antibody
  2. 2. Detection Antibody conjugated to HRP
  3. 3. Standards
  4. 4. Wash Buffer Concentrate
  5. 5. Dilution Buffer Concentrate
  6. 6. Color Reagent A
  7. 7. Color Reagent B
  8. 8. Stop Solution

Alternate Names for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
TNF RII/TNFRSF1B
[HRP]
DRT200
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
(76 Publications)
Species: Hu
Applications: ELISA
TXNDC15 Antibody
NBP1-89394
Immunocytochemistry/ Immunofluorescence TXNDC15 Antibody
Immunohistochemistry-Paraffin TXNDC15 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot CDP/CUTL1 Antibody (2A10)
Immunohistochemistry-Paraffin CDP/CUTL1 Antibody (2A10)
ELISA CDP/CUTL1 Antibody (2A10)
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
IL-2 R beta
Antibody (27302)
MAB224
Immunohistochemistry IL-2 R beta Antibody (27302)
Neutralization IL-2 R beta Antibody (27302)
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
LEDGF Antibody
(762815) [Unconjugate
MAB3468
Western Blot LEDGF Antibody (762815) [Unconjugated]
Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC, WB
HCLS1 Antibody
(1A8)
H00003059-M02
Western Blot HCLS1 Antibody (1A8)
Proximity Ligation Assay HCLS1 Antibody (1A8)
Immunohistochemistry-Paraffin HCLS1 Antibody (1A8)
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
KHSRP Antibody
NBP1-18910
Immunohistochemistry-Paraffin KHSRP Antibody
Immunohistochemistry-Paraffin KHSRP Antibody
Western Blot KHSRP Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Siglec-7/CD328
Antibody [Unconjugate
AF1138
Neutralization Siglec-7/CD328 Antibody [Unconjugated]
(1 Review)
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(663 Publications)
Species: Mu
Applications: ELISA
TNF RI/TNFRSF1A
[HRP]
DRT100
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
(86 Publications)
Species: Hu
Applications: ELISA
GRP78/HSPA5
Antibody - BSA Free
NBP1-06274
Flow Cytometry GRP78/HSPA5 Antibody - BSA Free
Flow Cytometry GRP78/HSPA5 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence GRP78/HSPA5 Antibody - BSA Free
(2 Reviews)
(44 Publications)
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
TPPP/p25 Antibody
NBP2-34031
Western Blot TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Protein Disulfide Isomerase/P4HB ELISA Kit (NBP2-80325) (0)

There are no publications for Protein Disulfide Isomerase/P4HB ELISA Kit (NBP2-80325).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protein Disulfide Isomerase/P4HB ELISA Kit (NBP2-80325) (0)

There are no reviews for Protein Disulfide Isomerase/P4HB ELISA Kit (NBP2-80325). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Protein Disulfide Isomerase/P4HB ELISA Kit (NBP2-80325) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Protein Disulfide Isomerase/P4HB Products

Research Areas for Protein Disulfide Isomerase/P4HB ELISA Kit (NBP2-80325)

Find related products by research area.

Blogs on Protein Disulfide Isomerase/P4HB

There are no specific blogs for Protein Disulfide Isomerase/P4HB, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Cell surface Protein Disulfide Isomerase/P4HB associated with GC-AC. Proteins were pulled down with anti GC-A antibody from HUVEC or HMC membrane preparations and detected with anti-Protein Disulfide Isomerase/P4HB antibody (RL90)compared to control IP (no anti GC-A) and non-immunoprecipitated proteins from HMC and HUVEC. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0112986), licensed under a CC-BY license.
Protein Disulfide Isomerase/P4HB Antibody (RL90)
NB300-517

Contact Information

Product PDFs

  Manual - Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol P4HB