Proteasome beta 1 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP1-89714] - Staining of human colon, kidney, liver and testis using Anti-PSMB1 antibody NBP1-89714 (A) shows similar protein ...read more
Independent Antibodies: Western Blot: Proteasome beta 1 Antibody [NBP1-89714] - Analysis using Anti-PSMB1 antibody NBP1-89714 (A) shows similar pattern to independent antibody NBP1-89713 (B).
Immunocytochemistry/ Immunofluorescence: Proteasome beta 1 Antibody [NBP1-89714] - Staining of human cell line Hep G2 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP1-89714] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP1-89714] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP1-89714] - Staining of human kidney shows moderate to strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP1-89714] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

Proteasome beta 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRKD
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Proteasome beta 1 Protein (NBP1-89714PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Proteasome beta 1 Antibody

  • EC 3.4.25.1
  • FLJ25321
  • HC5
  • KIAA1838
  • Macropain subunit C5
  • Multicatalytic endopeptidase complex subunit C5
  • PMSB1
  • proteasome (prosome, macropain) subunit, beta type, 1
  • proteasome beta 1 subunit
  • Proteasome beta 1
  • Proteasome component C5
  • Proteasome gamma chain
  • proteasome subunit beta type-1
  • proteasome subunit HC5
  • PSC5
  • PSMB1

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Proteasome beta 1 Antibody (NBP1-89714) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMB1