![Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Western-Blot-NBP2-02577-img0019.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI1D5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PFN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 14.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Profilin 1 Antibody (NBP2-02577)Find related products by research area.
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Profiling the Profilin 1 Antibody Profilin-1, or Pfn-1, is a small actin-binding protein which plays an essential role controlling the growth of microfilaments. Profilin 1 and Profilin 2 have similar biochemical properties but are expressed in different tissues. The Profilin 1 antibod... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunocytochemistry-Immunofluorescence-NBP2-02577-img0013.jpg "Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.")
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunohistochemistry-Paraffin-NBP2-02577-img0015.jpg "Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.")
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0011.jpg "Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then analyzed by flow cytometry.")
![Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Western-Blot-NBP2-02577-img0016.jpg "Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.")
![Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Western-Blot-NBP2-02577-img0017.jpg "Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.")
![Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Western-Blot-NBP2-02577-img0018.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).")
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunohistochemistry-Paraffin-NBP2-02577-img0014.jpg "Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.")
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0009.jpg "Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0012.jpg "Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416113530.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114766.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241613638.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114752.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241611472.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114785.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241613635.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416113538.jpg "Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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