PPP1R9B/Spinophilin Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Spinophilin, also known as Neurabin-2 and PPP1R9B, has 815 amino acids and is approximately 89kDa. Spinophilin is a scaffold protein that is involved in many signaling pathways, such as the cAMP pathway, the G-protein coupled receptor signaling pathway, the IL-6 pathway, and the Beta-Adrenergic signaling pathway. Spinophilin may be implicated in the linking of the plasma membrane to the actin cytoskeleton at synaptic junctions. Current research on Spinophilin is being performed in relation to several diseases and disorders including anorexia nervosa, Alzheimer's disease, schizophrenia, encephalitis, breast cancer, mood disorder, and glioblastoma. Spinophilin has also been shown to have interactions with CCKAR, CCKBR, ADRA1B, DRD2, and PPP1CA.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-05991

• PPP1R9B/Spinophilin Antibodies
• PPP1R9B/Spinophilin Lysate