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Podocalyxin Like Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Podocalyxin Like Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574
Specificity
Detects human Podocalyxin in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PODXL
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publications using
BAF1658 in the following applications:

  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody [Biotin]

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  1.  Li, J. et al. (2001) DNA Seq. 12:407.
  2.  Hara, T. et al. (1999) Immunity 11:567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podocalyxin Like Antibody (BAF1658)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(2)
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Human
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using BAF1658 Applications Species
Lim, SW;Na, D;Lee, H;Fang, X;Cui, S;Shin, YJ;Lee, KI;Lee, JY;Yang, CW;Chung, BH; Modeling of FAN1-Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System Cells 2023-09-20 [PMID: 37759541] (IHC, Human) IHC Human
S Cui, X Fang, H Lee, YJ Shin, ES Koh, S Chung, HS Park, SW Lim, KI Lee, JY Lee, CW Yang, BH Chung Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system Journal of Translational Medicine, 2023-02-22;21(1):138. 2/22/2023 [PMID: 36814269]
SW Lim, X Fang, S Cui, H Lee, YJ Shin, EJ Ko, KI Lee, JY Lee, BH Chung, CW Yang CRISPR-Cas9-Mediated Correction of SLC12A3 Gene Mutation Rescues the Gitelman's Disease Phenotype in a Patient-Derived Kidney Organoid System International Journal of Molecular Sciences, 2023-02-03;24(3):. 2023-02-03 [PMID: 36769335] (IHC, Human) IHC Human
Laura Amo, Javier Díez-García, Estíbaliz Tamayo-Orbegozo, Natalia Maruri, Susana Larrucea Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site Frontiers in Immunology 5/31/2022 [PMID: 35711462]
Garreta E, Prado P, Stanifer ML et al. A diabetic milieu increases ACE2 expression and cellular susceptibility to SARS-CoV-2 infections in human kidney organoids and patient cells Cell Metabolism 6/7/2022 [PMID: 35561674]
Garreta E, Prado P, Stanifer ML et al. A diabetic milieu increases ACE2 expression and cellular susceptibility to SARS-CoV-2 infections in human kidney organoids and patient cells Cell Metabolism 2022-06-07 [PMID: 35561674]
Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 4/1/2019 [PMID: 30778227]
Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 2019-04-01 [PMID: 30778227]

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Bioinformatics

Gene Symbol PODXL
Uniprot