Novus Biologicals products are now on bio-techne.com

Podocalyxin Like Antibody (CL0308)

Images

 
Western Blot: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Lane 1: Marker [kDa], Lane 2: Human kidney tissue lysate
Orthogonal Strategies: Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining in human kidney and liver tissues. Corresponding PODXL RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human fallopian tube shows moderate positivity in apical membranes of glandular cells.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human rectum shows weak to moderate membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL0308
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Podocalyxin Like Antibody (CL0308) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGD
Epitope
IHTKLPAKDVYERLK
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PODXL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Podocalyxin Like Recombinant Protein Antigen (NBP2-52912PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Podocalyxin Like Antibody (CL0308)

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-45312
Species: Hu, Pm, Mu(-)
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00001719-M01
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-32870
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
AF3159
Species: Mu
Applications: IHC
NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-64775
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, Func, IHC, IHC-Fr, IHC-P (-), IP
AF4117
Species: Rt
Applications: IHC, WB
664-LI
Species: Hu
Applications: BA
NBP3-07168
Species: Pm, Ca, Pm, Hu, Pm, Sq
Applications: Flow, ICC/IF
NBP3-35275
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
AF5866
Species: Hu
Applications: IHC, WB
NBP2-15104
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-14093
Species: Hu
Applications: IHC, IHC-P, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-52912
Species: Hu
Applications: WB, IHC

Publications for Podocalyxin Like Antibody (NBP2-52912) (0)

There are no publications for Podocalyxin Like Antibody (NBP2-52912).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Podocalyxin Like Antibody (NBP2-52912) (0)

There are no reviews for Podocalyxin Like Antibody (NBP2-52912). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Podocalyxin Like Antibody (NBP2-52912) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Podocalyxin Like Antibody (CL0308) and receive a gift card or discount.

Bioinformatics

Gene Symbol PODXL