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PLD2 Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10123) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLD2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of ...read more
PLD2 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human PLD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10123) at 10 µg/mL for 1 hour at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Host
Goat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF10123-SP
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PLD2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PLD2
Met1-His117
Accession # O14939
Specificity
Detects human PLD2 in direct ELISAs.
Source
N/A
Isotype
IgG
Host
Goat
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLD2 Antibody [Unconjugated]

  • Choline Phosphatase 2
  • HPLD2
  • PLD1C
  • PLD2

Background

Phospholipase D2 (PLD2) is an enzyme encoded by the PLD2 gene. Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. The Phospholipase D (PLD) lipid-signaling enzyme superfamily has long been studied for its roles in cell communication and a wide range of cell biological processes. Potent PLD2 inhibitors are available, like VU 0364739 hydrochloride (Tocris Cat. 4171) and ML 298 hydrochloride (Tocris Cat. 4895). Three different PLD2 isoforms -PLD2A, PLD2B and PLD2C- have been described, ranging from 38 to 105 KDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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