![Western Blot: PKR Antibody (S08-2C8) [NBP3-19517] - Western blot detection of PKR in K562, Hela cell lysates using NBP3-19517 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 74kDa.](http://images.novusbio.com/fullsize/PKR-Antibody-S08-2C8-Western-Blot-NBP3-19517-img0003.jpg "Western Blot: PKR Antibody (S08-2C8) [NBP3-19517] - Western blot detection of PKR in K562, Hela cell lysates using NBP3-19517 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 74kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S08-2C8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.3 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human PKR (Uniprot # P19525) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EIF2AK2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 74 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.3 mg/ml |
| Purity | Affinity purified |
![Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Western_Blot_5226.jpg)
![Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/16351.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for PKR Antibody (NBP3-19517)Find related products by research area.
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eIF2alpha - a regulator of global translation in response to cellular stress Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni... Read full blog post. |
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PKR - Mediating cellular stress responses through multiple signaling pathways Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EIF2AK2 |
![Immunocytochemistry/Immunofluorescence: PKR Antibody (S08-2C8) [NBP3-19517] - Immunofluorescence of PKR (green) in Hela cells using NBP3-19517 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/PKR-Antibody-S08-2C8-Immunocytochemistry-Immunofluorescence-NBP3-19517-img0001.jpg "Immunocytochemistry/Immunofluorescence: PKR Antibody (S08-2C8) [NBP3-19517] - Immunofluorescence of PKR (green) in Hela cells using NBP3-19517 at dilution 1/200, and DAPI (blue)")
![Immunohistochemistry-Paraffin: PKR Antibody (S08-2C8) [NBP3-19517] - Immunohistochemistry of PKR in paraffin-embedded Human Cholangiocarcinoma using NBP3-19517 at dilution 1/2](http://images.novusbio.com/fullsize/PKR-Antibody-S08-2C8-Immunohistochemistry-Paraffin-NBP3-19517-img0002.jpg "Immunohistochemistry-Paraffin: PKR Antibody (S08-2C8) [NBP3-19517] - Immunohistochemistry of PKR in paraffin-embedded Human Cholangiocarcinoma using NBP3-19517 at dilution 1/2")
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![Immunocytochemistry PKR Antibody (HL71/10) [Unconjugated]](https://images.novusbio.com/images2/PKR_MAB1980_Immunocytochemistry_9844.jpg)
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![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
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![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")