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PKMYT1 Antibody - Azide and BSA Free

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Western Blot: PKMYT1 Antibody [NBP3-16036] - Western blot analysis of extracts of various cell lines, using PKMYT1 antibody (NBP3-16036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: PKMYT1 Antibody [NBP3-16036] - Western blot analysis of extracts of various cell lines, using PKMYT1 antibody (NBP3-16036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-16036-20ul
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PKMYT1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 75-362 of human PKMYT1 (NP_004194.3). HQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKMYT1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500 - 1:1000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for PKMYT1 Antibody - Azide and BSA Free

  • DKFZp547K1610
  • EC 2.7.11.1
  • FLJ20093
  • Myt1 kinase
  • MYT1membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
  • protein kinase, membrane associated tyrosine/threonine 1

Background

PKMYT1 is encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PKMYT1 Antibody -
Azide and BSA Free
NBP3-16036
PKMYT1 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA

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Bioinformatics

Gene Symbol PKMYT1