PIK3R4 Antibody Summary
Immunogen |
This antibody was developed against a recombinant protein corresponding to amino acids: QLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLG |
Predicted Species |
Mouse (96%), Rat (93%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
PIK3R4 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
- Immunohistochemistry 1:50 - 1:200
- Immunohistochemistry-Paraffin 1:50 - 1:200
|
Application Notes |
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
Control Peptide |
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Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.2) and 40% Glycerol |
Preservative |
0.02% Sodium Azide |
Purity |
Immunogen affinity purified |
Alternate Names for PIK3R4 Antibody
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for PIK3R4 Antibody (NBP2-38276) (0)
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Product General Protocols
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Video Protocols
FAQs for PIK3R4 Antibody (NBP2-38276). (Showing 1 - 1 of 1 FAQ).
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I'm trying to detect PIK3R4 by NBP2-38276 antibody in HeLa cells induced autophagy by amino acid starvation. However, it doesn't go well. The protocol is followed : Fixation:PFA, 20min, RT, Permeabilization:50ug/mL Digitonin or 0.3% Triton X-100/0.1% BSA in PBS(-), 5min, RT, Blocking:3% or 5% BSA in PBS(-), 30-60min, RT, First antibody:1:50 dilution, 2hr at RT or O/N at 4C. Please show me a detailed protocol.
- Unfortunately, we have not tested our PIK3R4 antibody in amino acid starved cell lines, so we do not have a protocol that we can recommend. I would recommend searching the literature to see if there might be a publication that can offer you some additional information.The image on our site was obtained by staining U2-OS cells using the following protocol
Secondary Antibodies
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Isotype Controls
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