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PIK3R4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody [NBP2-38276] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: PIK3R4 Antibody [NBP2-38276] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PIK3R4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: QLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLG
Predicted Species
Mouse (96%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3R4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PIK3R4 Protein (NBP2-38276PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIK3R4 Antibody

  • EC 2.7.11.1
  • MGC102700
  • p150
  • phosphatidylinositol 3-kinase-associated p150
  • Phosphoinositide 3-kinase adaptor protein
  • phosphoinositide 3-kinase regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4, p150
  • PI3-kinase p150 subunit
  • PI3-kinase regulatory subunit 4
  • PIK3R4
  • VPS 15
  • VPS15

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIK3R4 Antibody (NBP2-38276) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PIK3R4 Antibody (NBP2-38276). (Showing 1 - 1 of 1 FAQ).

  1. I'm trying to detect PIK3R4 by NBP2-38276 antibody in HeLa cells induced autophagy by amino acid starvation. However, it doesn't go well. The protocol is followed : Fixation:PFA, 20min, RT, Permeabilization:50ug/mL Digitonin or 0.3% Triton X-100/0.1% BSA in PBS(-), 5min, RT, Blocking:3% or 5% BSA in PBS(-), 30-60min, RT, First antibody:1:50 dilution, 2hr at RT or O/N at 4C. Please show me a detailed protocol.
    • Unfortunately, we have not tested our PIK3R4 antibody in amino acid starved cell lines, so we do not have a protocol that we can recommend. I would recommend searching the literature to see if there might be a publication that can offer you some additional information.The image on our site was obtained by staining U2-OS cells using the following protocol

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIK3R4
Uniprot