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PIAS1 Antibody (4A4)

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ELISA: PIAS1 Antibody (4A4) [H00008554-M06] - Detection limit for recombinant GST tagged PIAS1 is approximately 0.03ng/ml as a capture antibody.
ELISA: PIAS1 Antibody (4A4) [H00008554-M06] - Detection limit for recombinant GST tagged PIAS1 is approximately 0.03ng/ml as a capture antibody.
Proximity Ligation Assay: PIAS1 Antibody (4A4) [H00008554-M06] - Analysis of protein-protein interactions between TP53 and PIAS1. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PIAS1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Func
Clone
4A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PIAS1 Antibody (4A4) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PIAS1 (NP_057250, 543 a.a. ~ 651 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISLD
Specificity
PIAS1 - protein inhibitor of activated STAT, 1 (4A4)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIAS1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Proximity Ligation Assay
  • Western Blot
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PIAS1 Antibody (4A4)

  • AR interacting protein
  • DDXBP1
  • DDXBP1MGC141878
  • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
  • DEAD/H box-binding protein 1
  • E3 SUMO-protein ligase PIAS1
  • GBPRNA helicase II-binding protein
  • GU/RH-II
  • Gu-binding protein
  • MGC141879
  • PIAS1
  • Protein inhibitor of activated STAT protein 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT-1
  • zinc finger, MIZ-type containing 3
  • ZMIZ3

Background

This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIAS1
Entrez
Uniprot