Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by ...read more
PI 3-Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at ...read more
PI 3-Kinase p85 beta Antibody (572001) [Unconjugated] Summary
Immunogen
E. coli-derived recombinant human PI 3‑Kinase p85 beta Lys516-Gly642 Accession # O00459
Specificity
Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha , rhPI
3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for PI 3-Kinase p85 beta Antibody (572001) [Unconjugated]
Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha , respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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