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PI 3-Kinase p85 beta Antibody (572001) [Unconjugated]

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by ...read more
PI 3-Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
572001
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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PI 3-Kinase p85 beta Antibody (572001) [Unconjugated] Summary

Specificity
Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha , rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB6777 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PI 3-Kinase p85 beta Antibody (572001) [Unconjugated]

  • P85B
  • p85-beta
  • phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
  • phosphatidylinositol 3-kinase regulatory subunit beta
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
  • phosphoinositide-3-kinase, regulatory subunit 2 (beta)
  • phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)
  • phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
  • PI 3Kinase p85 beta
  • PI 3-Kinase p85 beta
  • PI3K regulatory subunit beta
  • PI3-kinase regulatory subunit beta
  • PI3-kinase subunit p85-beta
  • PIK3R2
  • ptdIns-3-kinase regulatory subunit beta
  • ptdIns-3-kinase regulatory subunit p85-beta

Background

Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha , respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PI 3-Kinase p85 beta Antibody (MAB6777)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PI 3-Kinase p85 beta Antibody (MAB6777) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on PI 3-Kinase p85 beta

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Bioinformatics

Gene Symbol PIK3R2
Uniprot