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PHYH Antibody (1F2-5B9)

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Western Blot: PHYH Antibody (1F2-5B9) [H00005264-M01] - Analysis of PHYH expression in transfected 293T cell line by PHYH monoclonal antibody (M01), clone 1F2-5B9.Lane 1: PHYH transfected lysate(38.5 KDa).Lane 2: Non-transfected lysate.
Western Blot: PHYH Antibody (1F2-5B9) [H00005264-M01] - Analysis of PHYH expression in transfected 293T cell line by PHYH monoclonal antibody (M01), clone 1F2-5B9.Lane 1: PHYH transfected lysate(38.5 KDa).Lane 2: ...read more
Sandwich ELISA: PHYH Antibody (1F2-5B9) [H00005264-M01] - Detection limit for recombinant GST tagged PHYH is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1F2-5B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PHYH Antibody (1F2-5B9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PHYH (AAH29512, 1 a.a. ~ 338 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQLRAAARLQIVLGHLGRPSAGAVVAHPTSGTISSASFHPQQFQYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL
Localization
peroxisome
Specificity
PHYH - phytanoyl-CoA hydroxylase (Refsum disease)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PHYH
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PHYH Antibody (1F2-5B9)

  • LNAP1
  • PAHXLN1
  • peroxisomal
  • PhyH
  • PHYH1
  • phytanoyl-CoA 2-hydroxylase
  • phytanoyl-CoA hydroxylase (Refsum disease)
  • phytanoyl-CoA hydroxylase
  • RD

Background

This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically, this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PHYH
Entrez
OMIM
Uniprot