Novus Biologicals products are now on bio-techne.com

NNT Antibody

Images

 
Western Blot: NNT Antibody [NBP1-32109] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NNT protein stained by NNT antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse kidney. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.
Western Blot: NNT Antibody [NBP1-32109] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - SW480 xenograft, using NNT antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse stomach. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NNT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane, Multi-pass membrane protein, Matrix side
Predicted Species
Bovine (99%), Xenopus (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NNT
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (88%), Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for NNT Antibody

  • MGC126502
  • mitochondrial
  • nicotinamide nucleotide transhydrogenaseMGC126503
  • Pyridine nucleotide transhydrogenase

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4928
Species: Hu
Applications: CyTOF-ready, Flow, WB
NBP1-68766
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-57578
Species: Hu
Applications: WB
NBP1-87349
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-88036
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF1445
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
MAB6495
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-84450
Species: Hu, Mu
Applications: IHC-WhMt, IHC, IHC-P, WB
DKK300
Species: Hu
Applications: ELISA
NBP1-88866
Species: Hu
Applications: IHC, IHC-P
H00055835-M02
Species: Hu
Applications: ELISA, IP, S-ELISA, WB
H00002770-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP1-76544
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP3-09392
Species: Hu, Mu
Applications: WB
NBP1-20194
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32109
Species: Hu, Mu, Rt, Bv, Xp
Applications: WB, ICC/IF, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NNT Antibody (NBP1-32109) (0)

There are no publications for NNT Antibody (NBP1-32109).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NNT Antibody (NBP1-32109) (0)

There are no reviews for NNT Antibody (NBP1-32109). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NNT Antibody (NBP1-32109) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NNT Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NNT