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Phospholipase D2 Antibody

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Western Blot: Phospholipase D2 Antibody [NBP3-35105] - Western blot analysis of various lysates using Phospholipase D2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
Western Blot: Phospholipase D2 Antibody [NBP3-35105] - Western blot analysis of various lysates using Phospholipase D2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Phospholipase D2 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Phospholipase D2 (NP_002654.3).

Sequence:
MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLD2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:100 - 1:500
Theoretical MW
106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for Phospholipase D2 Antibody

  • Choline phosphatase 2
  • EC 3.1.4.4
  • hPLD2
  • Phosphatidylcholine-hydrolyzing phospholipase D2
  • phospholipase D2
  • PLD 2
  • PLD1C

Background

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-35105
Phospholipase D2 Antibody
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Bioinformatics

Gene Symbol PLD2