![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Analysis in human salivary gland and skeletal muscle tissues using NBP1-84969 antibody. Corresponding PHLDA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-29620238107..jpg "Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Analysis in human salivary gland and skeletal muscle tissues using NBP1-84969 antibody. Corresponding PHLDA1 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: RMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVE |
| Predicted Species | Rat (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PHLDA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
| Publication using NBP1-84969 | Applications | Species |
|---|---|---|
| Janus P, Mrowiec K et al. PHLDA1 Does Not Contribute Directly to Heat Shock-Induced Apoptosis of Spermatocytes. Int J Mol Sci 2019-12-30 [PMID: 31906015] (WB, IF/IHC, Mouse) | WB, IF/IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PHLDA1 |
![Western Blot: PHLDA1 Antibody [NBP1-84969] - PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock and indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Western-Blot-NBP1-84969-img0007.jpg "Western Blot: PHLDA1 Antibody [NBP1-84969] - PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock and indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] - Staining of human salivary gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84969-img0004.jpg "Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] - Staining of human salivary gland shows strong positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-296202381239..jpg "Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells")
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-29620238148..jpg "Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.")
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-296202381536..jpg "Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells as expected.")
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-296202381650..jpg "Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.")
![Immunohistochemistry: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse and subjected to heat shock after 2, 4, and 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids and condensing spermatids are marked with arrowhead and arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-2092023181151.jpg "Immunohistochemistry: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse and subjected to heat shock after 2, 4, and 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids and condensing spermatids are marked with arrowhead and arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. (a) Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse & subjected to heat shock after 2, 4, & 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids & condensing spermatids are marked with arrowhead & arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. (b) Detection of apoptotic DNA breaks (by TUNEL assay, red; DNA stained with DAPI, blue) in seminiferous tubules of untreated mice & after heat shock in vivo & indicated recovery time (2–6 h). (c) Detection of PHLDA1 by immunofluorescence (red; DNA stained with DAPI, blue) in cryptorchid testes 7 & 14 days after surgery (upper panel) & detection of apoptotic DNA breaks (by TUNEL assay, red) in corresponding tissues (bottom panel). Scale bar—50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-310202416114768.jpg "Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. (a) Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse & subjected to heat shock after 2, 4, & 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids & condensing spermatids are marked with arrowhead & arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. (b) Detection of apoptotic DNA breaks (by TUNEL assay, red; DNA stained with DAPI, blue) in seminiferous tubules of untreated mice & after heat shock in vivo & indicated recovery time (2–6 h). (c) Detection of PHLDA1 by immunofluorescence (red; DNA stained with DAPI, blue) in cryptorchid testes 7 & 14 days after surgery (upper panel) & detection of apoptotic DNA breaks (by TUNEL assay, red) in corresponding tissues (bottom panel). Scale bar—50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-310202416114735.jpg "Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-310202416114722.jpg "Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry EGR1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2818_human-egr1-affinity-purified-polyclonal-ab-immunocytochemistry-3102024831..jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Immunocytochemistry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images2/Nestin_MAB1259_Immunocytochemistry_6561.jpg)
![Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/antibody/mab1259_human-nestin-mab-clone-196908-intracellular-staining-by-flow-cytometry-24120239959.jpg)
![Western Blot Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/mab1259_human-nestin-mab-clone-196908-41202410481129.jpg)
![N/A IGFBP-3 [HRP]](https://images.novusbio.com/images2/DATA_IGFBP3_DGB300_ELISA_694.jpg)
![N/A IGFBP-3 [HRP]](https://images.novusbio.com/images2/IGFBP-3_DGB300_ELISA_104.jpg)
![N/A Thrombospondin-1 [HRP]](https://images.novusbio.com/images2/Thrombospondin-1_DTSP10_ELISA_199.jpg)
![Western Blot Raf-1 Antibody (563002) [Unconjugated]](https://images.novusbio.com/images2/Raf-1_MAB4540_Western_Blot_9426.jpg)
![Immunohistochemistry Raf-1 Antibody (563002) [Unconjugated]](https://images.novusbio.com/images2/Raf-1_MAB4540_Immunohistochemistry_9891.jpg)
![Simple Western Raf-1 Antibody (563002) [Unconjugated]](https://images.novusbio.com/images2/mab4540_human-mouse-rat-raf-1-mab-clone-563002-simple-western-158202212919.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg)
![Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")