![Knockout Validated: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0003.jpg "Knockout Validated: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Rt, Mu, Po, PmSpecies Glossary |
| Applications | WB, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary. |
| Predicted Species | Mouse (96%), Porcine (90%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PHGDH |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-19801 | Applications | Species |
|---|---|---|
| Samanta D, Park Y, Andrabi SA et al. PHGDH Expression is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance and Lung Metastasis. Cancer Res. 2016-06-08 [PMID: 27280394] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for PHGDH Antibody (NBP2-19801)Find related products by research area.
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![Western Blot: PHGDH Antibody [NBP2-19801] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0001.jpg "Western Blot: PHGDH Antibody [NBP2-19801] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801] - Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody [N3C2], Internal diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Frozen-NBP2-19801-img0004.jpg "Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801] - Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody [N3C2], Internal diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP2-19801] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP2-19801-img0002.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP2-19801] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.")
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![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_788.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_789.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
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![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")