![Knockout Validated: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0013.jpg "Knockout Validated: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary. |
| Predicted Species | Porcine (97%), Chimpanzee (100%), Bovine (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PHGDH |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PHGDH Antibody (NBP1-32201)Find related products by research area.
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![Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0007.jpg "Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - PHGDH antibody detects PHGDH protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0014.jpg "Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - PHGDH antibody detects PHGDH protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0008.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.")
![Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0006.jpg "Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - Analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0012.jpg "Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - Analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0005.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.")
![Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (NBP1-32201). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunoprecipitation-NBP1-32201-img0011.jpg "Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (NBP1-32201). EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")