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PGAM5 Antibody

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Western Blot: PGAM5 Antibody [NBP3-27905] - Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP3-27905] - Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at ...read more
Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP3-27905] - Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with at 10 u/mL, followed by goat anti-rabbit IgG secondary ...read more
Western Blot: PGAM5 Antibody [NBP3-27905] - Loading: 15 u of lysates per lane. Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: PGAM5 Antibody [NBP3-27905] - Loading: 15 u of lysates per lane. Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: PGAM5 Antibody [NBP3-27905] - Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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PGAM5 Antibody Summary

Immunogen
PGAM5 antibody was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5.
Clonality
Polyclonal
Host
Rabbit
Gene
PGAM5
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10 - 20 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PGAM5 Antibody

  • Bcl-XL-binding protein v68
  • BXLBv68
  • EC 3.1.3.16
  • MGC5352
  • phosphoglycerate mutase family member 5BXLBV68
  • serine/threonine-protein phosphatase PGAM5, mitochondrial

Background

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate fora Keap1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PGAM5
Uniprot