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Perilipin Antibody

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Western Blot: Perilipin Antibody [NBP3-23511] - (0.3ug/ml) staining of Human Adipose tissue lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: Perilipin Antibody [NBP3-23511] - (0.3ug/ml) staining of Human Adipose tissue lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Perilipin Antibody Summary

Immunogen
Peptide with sequence RVSDSFFRPSVMEPC, from the C Terminus of the protein sequence according to NP_002657.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLIN1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
Western blot: Approx 55-58kDa band observed in Human Adipose tissue lysates (calculated MW of 56.0kDa according to Human NP_002657.3). Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin Antibody

  • PERI
  • perilipin 1
  • Perilipin
  • perilipin-1
  • PLIN
  • PLIN1
  • PLINLipid droplet-associated protein

Background

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PLIN1