![Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK-1 (C-terminal region) mouse monoclonal antibody or anti-PDK-1 (Tyr-9) (NBP3-23292) rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/nbp3-23292_rabbit-pdk-1-p-tyr9-pab-19920231992816.jpg "Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK-1 (C-terminal region) mouse monoclonal antibody or anti-PDK-1 (Tyr-9) (NBP3-23292) rabbit polyclonal antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Phospho-PDK-1 (Tyr-9) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 9 in human PDK-1. This sequence is highly conserved in rat and mouse PDK-1. [UniProt# O15530] |
| Modification | p Tyr9 |
| Specificity | This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK-1 (Tyr-9) peptide. The purified antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDPK1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | PDPK1 |
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