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PDK-1 Antibody (M146)

Images

 
Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody (M146) [NBP3-23291] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse ...read more
Western Blot: PDK-1 Antibody (M146) [NBP3-23291] - Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK-1 (C-terminal region) (NBP3-23291).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
M146
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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PDK-1 Antibody (M146) Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
Clone M146 was generated from a recombinant protein containing amino acid residues in the C-terminal region of human PDK-1. This sequence is highly conserved in rat and mouse PDK-1. [UniProt# O15530]
Specificity
The antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK-1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDPK1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:250
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for PDK-1 Antibody (M146)

  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide-dependent protein kinase 2 pseudogene
  • PDK1
  • PDK-1
  • PDPK1
  • PDPK2
  • PDPK2P
  • PkB kinase like gene 1
  • PKB kinase

Background

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDK-1 Antibody (NBP3-23291) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDPK1