![Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0005.jpg "Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 339 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant PCNA interdomain connector loop protein |
| Localization | Nuclear |
| Marker | Proliferation Marker |
| Specificity | This reacts with the interdomain connector loop of human PCNA, other not determined. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PCNA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Predicted MW is 31.32 kDa. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
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| Publication using NB100-478 | Applications | Species |
|---|---|---|
| Malkas L H, Herbert B S, Abdel-Aziz W et al. A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. PNAS; 103; 19472-19477. 2006-01-01 [PMID: 17159154] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PCNA Antibody (NB100-478)Find related products by research area.
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The dynamic use of a PCNA antibody in fish, porcine and primate species Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair. Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl... Read full blog post. |
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The relationship between Ki67 and HIF-1 in cancer Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life. First discovered to be located to dividing cells,... Read full blog post. |
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Caspase 3 - a Reliable Marker for Index of Apoptosis Induction Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T... Read full blog post. |
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PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein) PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl... Read full blog post. |
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PCNA Antibodies: Marking Cell Proliferation & DNA Replication Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ... Read full blog post. |
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PCNA is a Universal Marker of Proliferating Cells Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital... Read full blog post. |
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Using PCNA as an Antibody Marker PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: PCNA Antibody (339) [NB100-478] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using 1:500 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Immunocytochemistry-Immunofluorescence-NB100-478-img0002.jpg "Immunocytochemistry/Immunofluorescence: PCNA Antibody (339) [NB100-478] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using 1:500 dilution.")
![Western Blot: PCNA Antibody (339) [NB100-478]](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0001.jpg "Western Blot: PCNA Antibody (339) [NB100-478]")
![Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat , B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0003.jpg "Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat , B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG")
![Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0004.jpg "Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")