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PCK2 Antibody

Images

 
Independent Antibodies: Western Blot: PCK2 Antibody [NBP2-33606] - Analysis using Anti-PCK2 antibody NBP2-33606 (A) shows similar pattern to independent antibody NBP2-33685 (B).
Immunocytochemistry/ Immunofluorescence: PCK2 Antibody [NBP2-33606] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human liver.
Immunohistochemistry: PCK2 Antibody [NBP2-33606] - Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human pancreas shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining in human duodenum and pancreas tissues using anti-PCK2 antibody. Corresponding PCK2 RNA-seq data are presented for the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human cerebral cortex, colon, kidney and liver using Anti-PCK2 antibody NBP2-33606 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human colon.
Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human kidney.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PCK2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PCK2 Protein (NBP2-33606PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PCK2 Antibody

  • EC 4.1.1.32
  • PE
  • PEPCK
  • PEPCK2PEP carboxykinase
  • PEPCK-M
  • phosphoenolpyruvate carboxykinase [GTP], mitochondrial
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
  • Phosphoenolpyruvate carboxylase
  • phosphopyruvate carboxylase

Background

PCK2 encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PCK2 Antibody (NBP2-33606) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCK2
Uniprot