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PBEF/Visfatin/NAMPT Antibody (362616) [Unconjugated]

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3T3‑L1 mouse embryonic fibroblast adipose‑like cell line was stained with Rat Anti-Human/Mouse PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4044, filled histogram) or isotype control antibody (Catalog # MAB006, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
362616
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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PBEF/Visfatin/NAMPT Antibody (362616) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PBEF/Visfatin
Met1-His491
Accession # Q99KQ4
Specificity
Detects human and mouse PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human PBEF is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
NAMPT
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publication using MAB4044.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PBEF/Visfatin/NAMPT Antibody (362616) [Unconjugated]

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

PBEF, also called Nampt or visfatin, is a ubiquitous 52 kDa nicotinamide phosphoribosyltransferase. It is the rate-limiting component in the biosynthesis of NAD+, and functions in the cytoplasm to regulate energy metabolism during stress responses and immune activation. Although it lacks a signal sequence, PBEF appears to be secreted by visceral adipose tissue and functions as a noncompetitive insulin mimetic. Mouse PBEF shows 96% and > 99% aa identity with human and rat PBEF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PBEF/Visfatin/NAMPT Antibody (MAB4044)(1)

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Bioinformatics

Gene Symbol NAMPT
Uniprot