![Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:1000.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT722-Western-Blot-NBP3-13562-img0006.jpg "Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | GT722 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118). The exact sequence is proprietary. |
| Predicted Species | Chicken (100%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PXN |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | PXN |
![Immunocytochemistry/Immunofluorescence: Paxillin Antibody (GT722) [NBP3-13562] - Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT722-Immunocytochemistry-Immunofluorescence-NBP3-13562-img0004.jpg "Immunocytochemistry/Immunofluorescence: Paxillin Antibody (GT722) [NBP3-13562] - Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.")
![Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT722-Western-Blot-NBP3-13562-img0001.jpg "Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT722-Western-Blot-NBP3-13562-img0002.jpg "Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT722-Western-Blot-NBP3-13562-img0003.jpg "Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT722-Western-Blot-NBP3-13562-img0005.jpg "Western Blot: Paxillin Antibody (GT722) [NBP3-13562] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (NBP3-13562) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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