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Park7/DJ-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was ...read more
Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) at 10 µg/mL for 3 ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse ...read more
Park7/DJ‑1 was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) at 15 µg/mL for ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price
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Park7/DJ-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Park7/DJ-1
Ala2-Asp189
Accession # Q99LX0
Specificity
Detects human and mouse Park7/DJ-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PARK7
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
AF3668 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Park7/DJ-1 Antibody [Unconjugated]

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease. Human and mouse Park7 share 92% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Park7/DJ-1 Antibody (AF3668)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Park7/DJ-1 Antibody (AF3668) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Park7/DJ-1 Products

Blogs on Park7/DJ-1.

Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.

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Bioinformatics

Gene Symbol PARK7
Uniprot