Park7/DJ-1 Antibody (4H4) [Alexa Fluor® 405] Summary
Immunogen |
Full length recombinant human Park7(DJ-1) expressed in and purified from E. coli. [UniProt# Q99497] |
Localization |
Cytoplasm. Nucleus. Mitochondrion. Note: Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
PARK7 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Knockout Validated
- Simple Western
- Western Blot
|
Application Notes |
This Park7(DJ-1), clone 4H4, antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 21 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Not reactive with rodent.
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
50mM Sodium Borate |
Preservative |
0.05% Sodium Azide |
Purity |
Protein G purified |
Notes
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Park7/DJ-1 Antibody (4H4) [Alexa Fluor® 405]
Background
PARK7, also known as DJ-1, is a member of the peptidase C56 protein family. The PARK-7 protein is a positive regulator of androgen receptor-dependent transcription. It has also been reported to be a potential sensor for oxidative stress, a redox-sensitive chaperone, and a key protein for preventing neuronal oxidative stress and cell death.
PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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