![Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Staining of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-007-Immunocytochemistry-Immunofluorescence-NBP3-05805-img0001.jpg "Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Staining of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | 007 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human Park7/DJ-1 (Uniprot#: Q99497-1; Met1-Asp189). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PARK7 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Park7/DJ-1 Antibody (NBP3-05805)Find related products by research area.
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Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease? The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug... Read full blog post. |
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| Gene Symbol | PARK7 |
![Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Analysis of Human PARK7 expression on HeLa cells. The cells were treated, stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-007-Flow-Cytometry-NBP3-05805-img0002.jpg "Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Analysis of Human PARK7 expression on HeLa cells. The cells were treated, stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PARK7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARK7 rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-007-Immunoprecipitation-NBP3-05805-img0004.jpg "Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PARK7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARK7 rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")