pan Actin Antibody (5J11)

Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian Actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42 kDa, and one or more actins are found in essentially every type of crude cellular and tissue extract. As a result, antibodies to Actin are widely used. As western blotting standards, they can be used to verify that the various steps of the western blotting procedure have been performed correctly. In addition, Actin is regarded as a "housekeeping" protein which is generally not altered much in expression as a result of experimental manipulations. Thus, quantitation of the Actin band on the western is used as a standard against the band density of other proteins. The Actin isotypes were originally classified as alpha, beta and gamma forms based on their charges which resulted in different mobilities in the isoelectric focusing dimension as seen on 2-dimensional SDS-PAGE. Subsequently, the alpha spot was found to potentially contain three Actin gene products, alpha-skeletal Actin, alpha-vascular smooth muscle Actin, and alpha-cardiac muscle. The beta spot contained a single protein called simply beta Actin, while the gamma spot may contain both gamma-1 Actin and gamma-2, which are enteric and smooth muscle Actins. The six mammalian gene products are very similar as shown by a sequence alignment of the human proteins. The most closely related actin isotypes are 97% identical, while the most distantly related are 94% identical, with most of the variability at the N-terminus. With this level of identity, antibodies to any one protein may recognize all six isotypes, as is the case with clone 5J11. This antibody binds all six isotypes very strongly on western blots, and it is a very effective blotting standard which can work on any cell type or tissue extract. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in Actin.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 3 applications: Block/Neutralize, In vivo assay, WB.

Showing Publications 1 - 2 of 2.

Publications using NBP2-25142	Applications	Species
Deissler HL, Rehak M, Busch C, Wolf A Blocking of VEGF-A is not sufficient to completely revert its long-term effects on the barrier formed by retinal endothelial cells Experimental eye research 2022-01-14 [PMID: 35038456] (WB, Bovine)	WB	Bovine
J�ckle A, Ziemssen F, Kuhn EM et al. Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure Journal of Diabetes Research 2020-05-26 [PMID: 32566677] (In vivo assay, Block/Neutralize)	In vivo assay, Block/Neutralize

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Alissa Taylor	WB	Human	09/24/2014	View Summary Application Western Blot Sample Tested Hela whole cell lysate Species Human
Enlarge	5 reviewed by: Verified Customer	WB	Human	06/17/2014	View Summary Application Western Blot Sample Tested 293T and HepG2 lysates Species Human

Array NBP2-25142

• pan Actin Antibodies