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PAK2 Antibody (677411)

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Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAK2 Monoclonal Antibody (Catalog # MAB6849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAK2 Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
677411
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6849-SP
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PAK2 Antibody (677411) Summary

Immunogen
E. coli-derived recombinant human PAK2
Leu127-Gln221
Accession # Q13177
Specificity
Detects human PAK2 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) PAK1 and no cross-reactivity with rhPAK1B, 3, 4, 6, or 7 is observed.
Source
N/A
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
PAK2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAK2 Antibody (677411)

  • EC 2.7.11
  • gamma-PAK
  • p21 (CDKN1A)-activated kinase 2
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • p21-activated kinase 2
  • p58
  • PAK2
  • PAK-2
  • PAK65
  • PAK65EC 2.7.11.1
  • PAKgamma
  • S6/H4 Kinase
  • serine/threonine-protein kinase PAK 2

Background

PAK2 (p21-activated kinase-2) is a widely expressed 58-62 kDa cytoplasmic ser/thr kinase of the PAK family, with substrates including ribosomal S6 and histone H4 proteins. The 524 amino acid (aa) human PAK2 contains phosphorylation, acetylation (K38, K128) and myristoylation (G213) sites, a CRIB (cdc42/Rac interactive binding) domain (aa 74-87), a caspase-3 cleavage site after aa 212, and a kinase domain (aa 249-499). Full-length PAK2 stimulates cell growth and motility, while the caspase cleaved PAK2 kinase domain (34 kDa) is involved in cell death. Within the region used as an immunogen, human PAK2 shares 87% aa identity with mouse and rat PAK2. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAK2
Uniprot