PABPN1 Antibody

Images

 
Western Blot: PABPN1 Antibody [NBP1-57216] - N-terminal region validated by WB using C2C12 myoblast cell line at 1.25 ug/ml.
Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody [NBP1-57216] - C2C12 myoblast cell line.
Western Blot: PABPN1 Antibody [NBP1-57216] - C2C12 myoblast cell line, Antibody Titration: 0.2-1 ug/ml

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PABPN1 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to PABPN1(poly(A) binding protein, nuclear 1) The peptide sequence was selected from the N terminal of PABPN1. Peptide sequence MAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGL. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PABPN1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1.0 ug/ml
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for PABPN1 Antibody

  • Nuclear poly(A)-binding protein 1
  • OPMD
  • PAB2poly(A) binding protein II
  • PABII
  • PABP-2
  • PABP2polyadenylate-binding protein 2
  • poly(A) binding protein 2
  • poly(A) binding protein, nuclear 1
  • Poly(A)-binding protein 2
  • Poly(A)-binding protein II
  • poly(A)-binding protein, nuclear 1
  • Polyadenylate-binding nuclear protein 1

Background

This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the pol

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PABPN1 Antibody (NBP1-57216) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PABPN1