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P4HA1 Antibody

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Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP2-19664] - Human lung cancer. P4HA1 stained by P4HA1 antibody [C1C3] diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP2-19664] - Human lung cancer. P4HA1 stained by P4HA1 antibody [C1C3] diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: P4HA1 Antibody [NBP2-19664] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with P4HA1 antibody [C1C3] (NBP2-19664) diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: P4HA1 Antibody [NBP2-19664] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with P4HA1 antibody [C1C3] (NBP2-19664) diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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P4HA1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human P4HA1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum lumen
Predicted Species
Mouse (97%), Rat (95%), Porcine (99%), Bovine (99%), Chicken (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HA1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for P4HA1 Antibody

  • 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • 2-oxoglutarate-4-dioxygenase subunit alpha-1
  • 4-PH Alpha-1
  • alpha polypeptide I
  • EC 1.14.11.2
  • P4HA
  • P4HA1
  • Procollagen-proline
  • prolyl 4-hydroxylase, alpha polypeptide I

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-19664
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Applications: WB, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol P4HA1
Uniprot