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P4HA2 Antibody

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Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Analysis in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Analysis in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.
Genetic Strategies: Western Blot: P4HA2 Antibody [NBP2-33878] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-P4HA2 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: P4HA2 Antibody [NBP2-33878] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human epididymis shows high expression.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Independent Antibodies: Analysis using NBP2-33878 (A) shows similar pattern to independent antibody NBP2-33909 (B).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

    

Genetic Strategies

  

Orthogonal Strategies

  

Order Details

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P4HA2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGM
Predicted Species
Mouse (90%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HA2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
P4HA2 Recombinant Protein Antigen (NBP2-33878PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for P4HA2 Antibody

  • 2-oxoglutarate-4-dioxygenase subunit alpha-2
  • 4-PH alpha 2
  • 4-PH alpha-2
  • alpha polypeptide II
  • collagen prolyl 4-hydroxylase alpha(II)
  • C-P4Halpha(II)
  • EC 1.14.11.2
  • Procollagen-proline
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • prolyl 4-hydroxylase subunit alpha-2
  • prolyl 4-hydroxylase, alpha polypeptide II

Background

P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol P4HA2
Uniprot