PHD4/HIF Prolyl Hydroxylase 4 Antibody

HIF prolyl 4-hydroxylases (HIF-P4Hs or PHDs) are proyl hydroxylase domain-containing enzymes (PHD1, PHD2, PHD3, and P4H-TM) that mediate key physiological responses to hypoxia by modulating HIF1 alpha levels. PHD member proteins have a wide but varied tissue expression and PHD4/HIF Prolyl Hydroxylase 4 (P4H-TM) is a novel member of this family that localize to ER membrane as single-pass type II membrane protein with its catalytic site inside the lumen. P4H-TM is expressed mainly in pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland tissues and gets induced upon hypoxic trigger. P4H-TM catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins and hydroxylates HIF1A at 'Pro-402'/'Pro-564' sites. P4H-TM has been proposed to function as a cellular oxygen sensor and, under normoxic conditions, it targets HIF through hydroxylation for proteasomal degradation via VHL/von Hippel-Lindau ubiquitination complex. Ectopic over-expression of P4H-TM in cultured neuroblastoma cells was found to reduce HIF-1alpha ODDD reporter levels, whereas its knockdown increased HIF-1alpha protein levels. HIF-P4Hs plays a critical role in several pathologies such as myocardial infarction, stroke, peripheral vascular disease, inflammation, diabetes, carcinogenesis and severe anemias.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 4 applications: EM, ICC/IF, IHC-P, WB.

Showing Publications 1 - 3 of 3.

Publications using NB100-295	Applications	Species
Klotzsche-von Ameln A, Prade I, Grosser M et al. Prolyl hydroxylase-4 overexpression stimulates tumor angiogenesis in mouse osteosarcoma via transforming growth factor-alpha. Mol Cancer Res. 2013-09-18 [PMID: 24048703] (WB, Human)	WB	Human
Henze AT, Riedel J, Diem T et al. Prolyl hydroxylases 2 and 3 act in gliomas as protective negative feedback regulators of hypoxia-inducible factors. Cancer Res;70(1):357-66. 2010-01-01 [PMID: 20028863]
Khan Z, Michalopoulos GK, Stolz DB. Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation. Am J Pathol 2006-10-01 [PMID: 17003483] (IHC-P, WB, ICC/IF, EM, Rat)	IHC-P, WB, ICC/IF, EM	Rat

Array NB100-295

• PHD4/HIF Prolyl Hydroxylase 4 Antibodies
• PHD4/HIF Prolyl Hydroxylase 4 Lysate
• PHD4/HIF Prolyl Hydroxylase 4 Proteins